Journal:

Article Title: Biochemical-Genetic Analysis and Distribution of DES-1, an Ambler Class A Extended-Spectrum ?-Lactamase from Desulfovibrio desulfuricans

doi: 10.1128/AAC.46.10.3215-3222.2002

Figure Lengend Snippet: Autoradiogram after Southern hybridization of whole-cell DNAs of D. desulfuricans strain ATCC 27774, 13 Desulfovibrio sp. isolates, and P. aeruginosa strain ATCC 27853 (negative control). DNAs were digested with restriction enzymes SacII (A) and NarI (B) and were subsequently hybridized with a probe consisting of a 964-bp fragment internal to blaDES-1.

Article Snippet: Whole-cell DNA of Pseudomonas aeruginosa strain ATCC 27853 was used as a negative control in hybridization experiments. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Antibiotic b MIC (μg/ml) ATCC 27774 D1 D3 c D4, c D5, c D6 c D8 to D14, D16 AMX 0.25 0.25 128 8 32-64 AMX + CLA d 0.25 0.25 1 0.25 32-64 TIC 2 2 128 16 256 TIC + CLA 2 2 4 2 256 CTX 2 1 16 4-8 128 CTX + CLA 1 1 8 2-4 128 CTX + SUL e 0.06 0.06 8 2 128 CLA 8 8 8 8 2-4 SUL 4 8 16 16 32 TZB 32 64 256 128 128 Gentamicin 16 16 8 8 8-16 Chloramphenicol 4 4 16 8 8 Tetracycline 2 2 2 1-4 4 Ciprofloxacin 0.5 0.5 1 0.5 0.5-64 Open in a separate window a Strain numbering is according to Lozniewski et al. ( 26 ). b AMX, amoxicillin; TIC, ticarcillin; CTX, cefotaxime; CLA, clavulanic acid; SUL, sulbactam; TZB, tazobactam. c β-Lactamase positive, as assessed by the results of the nitrocefin dish test. d Clavulanic acid at a fixed concentration of 2 μg/ml. e Sulbactam at a fixed concentration of 8 μg/ml.

Techniques: Hybridization, Negative Control